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Journal: Clinical Microbiology and Infection
Article Title: Multidrug resistant (MDR) Klebsiella pneumoniae clinical isolates: a zone of high heterogeneity (HHZ) as a tool for epidemiological studies
doi: 10.1111/j.1469-0691.2012.03886.x
Figure Lengend Snippet: Genomic comparison. A. The K. pneumoniae JHCK1, VA 360, and 1.53 optical maps and the restriction maps of K. pneumoniae strains NTUH-K2044, KCTC 2242, and MGH 78578 AflII obtained in silico were compared using the MapSolver 3.2.0 software. The blue and white regions represent matching and non-matching DNA fragments, respectively. The genomic comparison was done between strains JHCK1, 1.53, and MGH 78578 vs. strains VA360, KCTC 2242, and NTUH-K2044, a combination that maximized the detection of the HHZ region among all strains using the default settings of MapSolver v.3.2.0. Vertical lines represent AflII restriction sites. B. Zoom-in the HHZ region. C. Synteny among K. pneumoniae MGH 78578, KCTC 2242, and NTUH-K2044 strains at the HHZ regions. The genetic maps and comparison have been generated using the ACT [12] to visualize the Blastn output. Colinear regions are represented in red, colinear regions that have been inverted are represented in blue, and deletions/insertions are represented in white. The HHZs have been colored yellow in MGH 78578, KCTC 2242 and NTUH-K2044 strains, the coordinate numbers are 2648600..2807698, 3122007.. 3313398 and 3356452..3613107 respectively.
Article Snippet: Analyses were done comparing the Afl II restriction maps using the
Techniques: Comparison, In Silico, Software, Generated